Composite

Part:BBa_K2481108:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


MelA expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1390
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1242
    Illegal BsaI.rc site found at 547


Design Notes

the melA cds was assembled with an T7 promoter that can be induced with IPTG in E. coli BL21(DE3) cells, and included with an E. coli RBS and terminator.


Source

DNA synthesized by IDT based on the Rhizobium etli gene sequence.

References